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Experimental Programs for SILAC in Protein Interaction

December 8, 2015 | Author: | Posted in Science

Identifying MS and analyzing protein quantification is the main theory of this technology to judge the specific binding protein bait . When certain protein content in the experimental group and control group reaches the statistical difference, it can be sure that the bait proteins interact with proteins. Program one Taking normal cell line as experimental material. 1.Culturing cells with light and heavy chain amino respectively. When it is cultured to five and six generations, the same amount of cells should be mixed and proteins also should be extracted from it. 2.Extracting the light and heavy chains proteins, which should be processed …